LGALS3 Protein (OPPA01624)

Catalog No: OPPA01624 (Formerly GWB-135C30)

Size:10UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The Galectin-3 protein was lyophilized from a concentrated (1mg/ml) containing 10mM sodium phosphate buffer, pH-7.5 and 50mM NaCl. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized LGALS3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml.
  Product Introduction: Galectin-3 mediates with the alpha-3, beta-1 integrin the stimulation by cspg4 of endothelial cells migration. Galectin-3 plays an necessary part during the acquisition of vasculogenic mimicry and angiogenic properties associated with melanoma progression. LGALS3 overexpression is highly expressed in early stages of papillary carcinoma, and its expression intensity declines during tumor progression. Serum levels of LGALS3 are high in patients with thyroid malignancy but there is considerable overlap in serum LGALS3 concentrations between those with benign and malignant nodular thyroid disease. LGLAS3 takes part as an immune regulator to inhibit T-cell immune responses and promote tumor growth, as a result providing a new mechanism for tumor immune tolerance.
  Product Description: LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 250 amino acids and having a molecular mass of 26 kDa.The LGALS3 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Galectin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Galectin-3 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI.

Target Info

• Gene Symbol: LGALS3
• Alias Symbols: L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2
• NCBI Gene Id: 3958
• Protein Name: Galectin-3
• Description of Target: Recombinant Human Galectin-3
• Uniprot ID: P17931
• Protein Accession #: NP_002297.2
• Protein Size (# AA): Recombinant