LGALS3 ELISA Kit (Human) (OKBB00803)

Catalog No: OKBB00803

Size:96 wells

LGALS3 ELISA Kit (Human) (OKBB00803) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human Galectin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-3 has been precoated onto 96-well plates. Standards(E.coli, A2-I250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Galectin-3 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	212	1876	4230	205	1951	4307
  Standard deviation	16.74	76.91	334.17	19.88	115.1	374.7
  CV(%)	7.9	4.1	7.9	9.7	5.9	8.7

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human LGALS3 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human LGALS3 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human LGALS3 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	212	196	200	216	206	8.24	4%
  Sample 2	1876	2001	1983	1897	1939	53.64	2.7%
  Sample 3	4230	4475	3962	4238	4226	181.56	4.2%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
  O.D.	0.013	0.117	0.201	0.405	0.746	1.191	1.680	2.010

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Galectin-3
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A2-I250
• Description: For quantitative detection of human Galectin-3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: LGALS3
• Gene Full Name: lectin, galactoside-binding, soluble, 3
• Alias Symbols: 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, Galectin-3, GALIG, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, lectin, galactoside-binding, soluble, 3, LGALS2Mac-2 antigen, MAC2, Macrophage galactose-specific lectin
• NCBI Gene Id: 3958
• Protein Name: Galectin-3
• Description of Target: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
• Uniprot ID: P17931
• Protein Accession #: NP_002297.2
• Nucleotide Accession #: NM_002306.3