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Datasheets/Manuals | Printable datasheet for LGALS3 Antibody (OABB01542) |
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Tested Species Reactivity | Human, Mouse, Rat |
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Predicted Species Reactivity | Human |
Product Format | Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot |
Additional Information | Notes: WB: The detection limit for Galectin 3 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively. |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human Immunocytochemistry/Immunofluorescence: 2 ug/ml: Human Flow Cytometry: 1-3 ug/1x10^6 cells: Human |
Storage Buffer | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Galectin-3(LGALS3) detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM in Human;Mouse;Rat. |
Gene Symbol | LGALS3 |
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Gene Full Name | galectin 3 |
Alias Symbols | 35 kDa lectin;advanced glycation end-product receptor 3;carbohydrate-binding protein 35;CBP35;epididymis secretory sperm binding protein;GAL3;galactose-specific lectin 3;Galactoside-binding protein;GALBP;galectin-3;GALIG;IgE-binding protein;L31;L-31;laminin-binding protein;lectin L-29;lectin, galactoside-binding, soluble, 3;LGALS2;MAC2;MAC-2 antigen. |
NCBI Gene Id | 3958 |
Protein Name | Galectin-3 |
Description of Target | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. |
Uniprot ID | P17931 |
Molecular Weight | 26152 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "LGALS3 Antibody (OABB01542)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Human".
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How long will it take to receive "LGALS3 Antibody (OABB01542)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "LGALS3 Antibody (OABB01542)" provided in?
This item is provided in "Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "LGALS3 Antibody (OABB01542)"?
This target may also be called "35 kDa lectin;advanced glycation end-product receptor 3;carbohydrate-binding protein 35;CBP35;epididymis secretory sperm binding protein;GAL3;galactose-specific lectin 3;Galactoside-binding protein;GALBP;galectin-3;GALIG;IgE-binding protein;L31;L-31;laminin-binding protein;lectin L-29;lectin, galactoside-binding, soluble, 3;LGALS2;MAC2;MAC-2 antigen." in publications.
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What is the shipping cost for "LGALS3 Antibody (OABB01542)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "LGALS3 Antibody (OABB01542)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "LGALS3 Antibody (OABB01542)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "26152 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "LGALS3 Antibody (OABB01542)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "LGALS3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "LGALS3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "LGALS3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "LGALS3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "LGALS3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "LGALS3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.