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Catalog No: OCOA00751
Size:20UG
Price: $179.00
SKU
OCOA00751
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LGALS1 Protein Lysate (OCOA00751)

Datasheets/ManualsPrintable datasheet for LGALS1 Protein Lysate (OCOA00751)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionLGALS1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolLGALS1
Gene Full Namegalectin 1
Alias Symbols14 kDa laminin-binding protein;14 kDa lectin;beta-galactoside-binding lectin L-14-I;beta-galactoside-binding protein 14kDa;epididymis secretory sperm binding protein;gal-1;GAL1;galaptin;galectin-1;GBP;HBL;HLBP14;HPL;lactose-binding lectin 1;Lectin galactoside-binding soluble 1;lectin, galactoside-binding, soluble, 1;putative MAPK-activating protein PM12;S-Lac lectin 1.
NCBI Gene Id3956
Protein NameA0A384MR27, NM_002305, NP_002296, P09382
Description of TargetGalectin, Galectin-1, galectin-1 [Homo sapiens], Homo sapiens galectin 1 (LGALS1), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P09382
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_002296
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_002305
Molecular Weight14.7 kDa
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