LGALS1 ELISA Kit (Rat) (OKCD01147)

Catalog No: OKCD01147 (Formerly GWB-KBBLP8)

Size:96WELLS

Rat Lgals1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	83-93%	79-89%	92-104%	78-96%
  EDTA plasma(n=5)	88-104%	94-101%	99-105%	80-96%
  heparin plasma(n=5)	89-101%	90-104%	97-105%	94-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 1 (GAL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 1 (GAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 1 (GAL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Galectin 1 (GAL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-102	95
  EDTA plasma(n=5)	91-98	95
  heparin plasma(n=5)	84-96	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Lgals1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Lgals1 Lyophilized Standard: 2 x 40 ng
  • 100X Biotinylated Lgals1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Sensitivity: < 0.246 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Galectin 1 (GAL1). No significant cross-reactivity or interference between Galectin 1 (GAL1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Lgals1
• Gene Full Name: galectin 1
• Alias Symbols: 14 kDa lectin;beta-galactoside-binding lectin L-14-I;gal-1;galaptin;galectin-1;lactose-binding lectin 1;Lectin galactoside-binding soluble 1;lectin, galactose binding, soluble 1;lectin, galactoside-binding, soluble, 1;RL 14.5;S-Lac lectin 1.
• NCBI Gene Id: 56646
• Protein Name: Galectin-1
• Description of Target: Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
• Uniprot ID: P11762
• Protein Accession #: NP_002296
• Nucleotide Accession #: NM_002305.3
• Protein Size (# AA): 135