LGALS1 Chemi-Luminescent ELISA Kit (Human) (OKCD05536)

Catalog No: OKCD05536

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Galectin 1 (GAL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Galectin 1 (GAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Galectin 1 (GAL1) level in the sample or standard.
• ELISA Kit Range: 68.6-50,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LGALS1 Microplate: 96 Wells (12 x 8 Well strips)
  • LGALS1 Lyophilized Standard: 2
  • 100X LGALS1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 29.7pg/mL

Target Info

• Gene Symbol: LGALS1
• Gene Full Name: galectin 1
• Alias Symbols: 14 kDa laminin-binding protein;14 kDa lectin;beta-galactoside-binding lectin L-14-I;beta-galactoside-binding protein 14kDa;epididymis secretory sperm binding protein;gal-1;GAL1;galaptin;galectin-1;GBP;HBL;HLBP14;HPL;lactose-binding lectin 1;Lectin galactoside-binding soluble 1;lectin, galactoside-binding, soluble, 1;putative MAPK-activating protein PM12;S-Lac lectin 1.
• NCBI Gene Id: 3956
• Protein Name: Galectin-1
• Description of Target: Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
• Uniprot ID: P09382
• Protein Accession #: NP_002296.1
• Nucleotide Accession #: NM_002305.3