LF ELISA Kit (Bovine) (OKWB00114)

Catalog No: OKWB00114

Size:96 Wells

Bovine LF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bovine
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	89-96%	85-92%	85-101%	87-102%
  EDTA Plasma (n=5)	85-100%	88-96%	82-95%	87-101%
  Heparin Plasma (n=5)	83-100%	81-96%	80-89%	85-93%

• ELISA Kit Principle: Aviva Systems Biology Lf ELISA Kit (Bovine) (OKWB00114) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lf has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lf captured in the well.
• ELISA Kit Range: 1.563 - 100 ng/mL
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-101	92
  EDTA Plasma (n=5)	88-104	94
  Heparin Plasma (n=5)	86-97	92

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Lf Microplate: 96 Wells (12 x 8 Well strips)
  • Lf Lyophilized Standard: 2 x 100 ng
  • 100X Lf Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.938 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LF
• Alias Symbols: Lf
• NCBI Gene Id: 280846
• Protein Name: Lactoferrin
• Uniprot ID: B9VPZ5
• Protein Accession #: NP_851341
• Nucleotide Accession #: NM_180998