Catalog No: OKCD02277
Size:96WELLS
Price: $750.00
SKU
OKCD02277
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for Leukotriene C4 ELISA Kit (OKCD02277)
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)95-103%79-101%78-88%82-92%
EDTA plasma(n=5)79-95%80-90%80-93%93-101%
heparin plasma(n=5)80-96%98-105%95-102%94-102%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene C4 (LTC4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene C4 (LTC4) and unlabeled Leukotriene C4 (LTC4) (Standards or samples) with the pre-coated antibody specific to Leukotriene C4 (LTC4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample.
ELISA Kit Range370.4-30,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)85-10189
EDTA plasma(n=5)85-9996
heparin plasma(n=5)91-10497
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukotriene C4 (LTC4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukotriene C4 (LTC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Leukotriene C4 Microplate96 Wells (12 x 8 Well Strips)
Leukotriene C4 Lyophilized Standard2
100X Leukotriene C4 Biotin Complex120 uL
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Additional InformationCAS: 72025-60-6
PubChem: 5280493
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sensitivity142.5 pg/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolLTC4
Alias SymbolsLeukotriene C4; LTC4; Leukotriene C; Leukotriene C1; Leucotriene C4
Protein NameLeukotriene C4
Description of TargetLeukotriene C4 is the conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)
Write Your Own Review
You're reviewing:Leukotriene C4 ELISA Kit (OKCD02277)
Your Rating