Leptin Salamander Protein (OPPA01973)

Catalog No: OPPA01973 (Formerly GWB-2B5BB6)

Size:100UG

Product Info

• Predicted Species Reactivity: Salamanders
• Product Format: The Salamander Leptin was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Salamander Leptin in sterile 0.4% NaHCO3 pH-9 not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: By inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity in that test is similar to that of mouse leptin (see Salomon et al (2006) Protein Expression and Purifcation 47, 128 - 136).
  Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.104 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
  Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.104 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
  Product Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
  Product Description: Leptin Salamander Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of 16kDa. The Salamander Leptin is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Salamander Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Leptin should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 99.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the ten N - terminal amino acids was determined and was found to be Ala - Ile - Met - Val - Asp - Gln - Leu - Arg - Met - Asp.

Target Info

• Gene Symbol: sLeptin
• Alias Symbols: OB Protein, Obesity Protein, OBS, Obesity factor, Leptin, Leptin Salamander, Leptin Salamander Recombinant
• Description of Target: Recombinant Salamander Leptin
• Protein Size (# AA): Recombinant