Catalog No: OPPA00552 (Formerly GWB-51F5A0)
Size:5UG
Price: $75.00
SKU
OPPA00552
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OPPA00552 |
---|
Predicted Species Reactivity | Human |
---|---|
Product Format | The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Host | E. Coli |
Application | WB, Protein Assay |
Additional Information | Solubility: The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point. |
:: | Product Introduction: Leptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately~16 kDa in mass and encoded by the obese (ob)gene. leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cellsin the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. |
Reconstitution and Storage | Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0C. Reconstituted Leptin is best stored refrigerated at 4C. Please avoid freeze-thaw cycles. |
Purity | Greater than 90.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Application Info | 1. Positive control for Western blot 2. Antibody production 3. Protein assay. |
Gene Symbol | Leptin His |
---|---|
Alias Symbols | OB, OBS, LEPD |
NCBI Gene Id | 3952 |
Protein Name | Leptin |
Description of Target | Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 48-167 and having a total molecular mass of 19 kDa including the 4 kDa His tag.The Leptin is purified by proprietary chromatographic techniques. |
Uniprot ID | P41159 |
Protein Accession # | NP_000221.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review