Leptin Protein (OPPA00493)

Catalog No: OPPA00493 (Formerly GWB-22EFDB)

Size:200UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.
  Biological Activity: Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.878 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.878 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.
  Product Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
  Product Description: Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa.The Leptin is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0C. Reconstituted Leptin is best stored refrigerated at 4C.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Val - Pro - Ile - Gln.

Target Info

• Gene Symbol: Leptin
• Alias Symbols: OB, OBS, LEPD
• NCBI Gene Id: 3952
• Protein Name: Leptin
• Description of Target: Recombinant Human Leptin
• Uniprot ID: P41159
• Protein Accession #: NP_000221.1
• Protein Size (# AA): Recombinant