Leptin Protein (OPPA00492)

Catalog No: OPPA00492 (Formerly GWB-F9D820)

Size:200UG

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Lyophilized from 0.0045mM NaHCO₃.
  Physical Appearance: Freeze-dried powder
• Host: E. Coli
  Protein quantitation was carried out by two independent methods:
  1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
  2. Analysis by RP-HPLC, using a calibrated solution of Leptin rat as a Reference Standard.
• Reconstitution and Storage: It is recommended to reconstitute the lyophilized Leptin in sterile 18M Omega-cm H₂O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Leptin should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purification: Purified by proprietary chromatographic techniques.
• Purity: Greater than 95.0% as determined by (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
• Biological Activity: Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
• Peptide Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-His.

Target Info

• Gene Symbol: rLeptin
• Alias Symbols: OB, obese
• NCBI Gene Id: 25608
• Protein Name: Leptin
• Description of Target: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. Leptin Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids.
• Uniprot ID: P50596
• Protein Accession #: NP_037208.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 16 kDa