LEPR ELISA Kit (Pig) (OKEH07273)

Catalog No: OKEH07273

Size:96T

Pig LEPR ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	84-84% (n = 20)	82-93% (n = 20)	81-92% (n = 20)	84-96% (n = 20)
  EDTA plasma(n=5)	100-109% (n = 20)	90-100% (n = 20)	110-120% (n = 20)	97-106% (n = 20)
  heparin plasma(n=5)	103-112% (n = 20)	90-101% (n = 20)	105-115% (n = 20)	100-110% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology LEPR ELISA Kit (Pig) (OKEH07273) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LEPR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LEPR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LEPR captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.5% (n = 20)
  Mean Inter-assay CV%: <=5.2% (n = 20)
• ELISA Kit Component:
  • LEPR Microplate: 96 Wells (12 x 8 Well strips)
  • LEPR Lyophilized Standard: 2
  • 100X Biotinylated LEPR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Receptor for hormone LEP/leptin (By similarity). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (By similarity). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T-cells. Leptin increases Th1 and suppresses Th2 cytokine production.
  Subcellular Location: Cell membrane Single-pass type I membrane protein Basolateral cell membrane
  Unigene: Ssc.263
  String: 9823.ENSSSCP00000022838
  KEGG: ssc:396836
  Pfam: PF06328
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.15ng/mL
• Specificity: Natural and recombinant Pig Leptin receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LEPR
• Gene Full Name: leptin receptor
• Alias Symbols: LEP-R;leptin receptor;OB receptor;OB-R.
• NCBI Gene Id: 396836
• Protein Name: O02671
• Description of Target: Leptin receptor
• Uniprot ID: https://www.uniprot.org/uniprot/O02671
• Protein Accession #: NP_001019758.1
• Nucleotide Accession #: NM_001024587.1