Catalog No: OKEH04548
Size:96T
Price: $525.00
SKU
OKEH04548
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Lepr ELISA Kit (Mouse) (OKEH04548) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lepr has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Lepr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lepr captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Lepr Microplate96 Wells (12 x 8 Well Strips)
Lepr Lyophilized Standard2
Sample Diluent1 x 20 mL
Lepr Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.163 ng/mL
SpecificityNatural and recombinant Mouse Leptin receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLepr
Gene Full Nameleptin receptor
Alias SymbolsB219;db;diabetes;LE;LEP-R;Leprb;LEPROT;leptin receptor;leptin receptor gene-related protein;Modb;Modb1;O;OB-;OB receptor;obese-;obese-like;obl;Obr;OB-RGRP.
NCBI Gene Id16847
Protein NameP48356
Description of TargetLeptin receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P48356
Protein Accession #NP_001116371.1
Nucleotide Accession #NM_001122899.1
Protein Size (# AA)1162
Molecular Weight131 KDa
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