LEP ELISA Kit (Human) (OKIA00213)

Catalog No: OKIA00213

Size:1 x 96 well plate

Representative Human LEP Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Human
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 130 Minutes
• ELISA Kit Principle: The Aviva Systems Biology LEP ELISA Kit (Human) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Leptin in Plasma or Serum. This assay employs an antibody specific for Human LEP coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-LEP antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LEP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of LEP in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 0.31 - 20 ng/mL
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Human LEP Microplate: 96 Wells (12 x 8 Well Strips)
  • Human LEP Calibrator: 1
  • 100X LEP Detection Antibody: 150 uL
  • 100X Streptavidin-HRP Conjugate: 150 uL
  • 5X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The LEP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring LEP/Leptin in Human biological samples.
  Sample Volume: 100 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 0.22 ng/mL

Target Info

• Alias Symbols: LEP;Leptin;Obese protein;OB;OBS;Obesity factor;
• Protein Name: Leptin
• Description of Target: Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (PubMed:17344214, PubMed:15899045, PubMed:19688109). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity) (PubMed:8589726, PubMed:11460888, PubMed:19688109, PubMed:24340098, PubMed:25060689).
• Uniprot ID: P41159