Catalog No: OKAA00022-96W
Size:96 Wells
Price: $425.00
SKU
OKAA00022-96W
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | ||||||||||||
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Application | ELISA | ||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||
ELISA Kit Reproducibility | The coefficient of variation of both intra-assay and inter-assay were less than 10%. | ||||||||||||
ELISA Kit Component |
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Additional Information | Sample Type: cell culture supernates, serum, and plasma | ||||||||||||
:: | Reagents: REAGENTS 1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human Leptin (8x12) 2. 2 vials human Leptin Standard lyophilized, 2000 pg/ml upon reconstitution 3. 2 vials concentrated Biotin-Conjugate anti-human Leptin monoclonal antibody 4. 2 vials Streptavidin-HRP solution, 5. 1 bottle Standard /sample Diluent 6. 1 bottle Biotin-Conjugate antibody Diluent 7. 1 bottle Streptavidin-HRP Diluent 8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 9. 1 vial Substrate Solution 10. 1 vial Stop Solution 11. 3 pieces Adhesive Films 12. package insert | ||||||||||||
Sample Type | cell culture, supernates, serum, and plasma | ||||||||||||
Sensitivity | 15 pg/ml | ||||||||||||
Predicted Homology Based on Immunogen Sequence | Human | ||||||||||||
Specificity | This assay recognizes both natural and recombinant human IL-32alpha. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed. Recombinant human: IL-1a, IL-1ra, IL-1 sRI, IL-1 sRII Recombinant mouse: IL-1a Recombinant porcine: IL-1b | ||||||||||||
Publications | Association between obstructive sleep apnea severity and endothelial dysfunction in patients with type 2 diabetes. Cardiovasc Diabetol. 16, 39 (2017). 28327146 | ||||||||||||
Assay Info | Assay Method: Quantitative Sandwich ELISA |
Gene Symbol | LEP |
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Gene Full Name | leptin |
Alias Symbols | OB, OBS, LEPD |
NCBI Gene Id | 3952 |
Protein Name | Leptin |
Description of Target | This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. |
Uniprot ID | P41159 |
Protein Accession # | NP_000221 |
Nucleotide Accession # | NM_000230 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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