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Catalog No: OKCD03710
Size:96WELLS
Price: $875.00
SKU
OKCD03710
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LEFTY1 Chemi-Luminescent ELISA Kit (Rat) (OKCD03710)

Datasheets/ManualsPrintable datasheet for LEFTY1 Chemi-Luminescent ELISA Kit (Rat) (OKCD03710)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)86-97%97-104%96-103%86-94%
EDTA Plasma (n=5)88-103%95-103%85-99%94-102%
Heparin Plasma (n=5)91-99%98-105%79-93%84-94%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)85-9389
EDTA Plasma (n=5)99-105102
Heparin Plasma (n=5)90-9793
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Lefty1 Microplate96 Wells (12 x 8 Well strips)
Lefty1 Lyophilized Standard2
100X Lefty1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLefty1
Gene Full Nameleft right determination factor 1
Alias Symbolsleft-right determination factor 1;RGD1561867.
NCBI Gene Id498299
Protein NameLeft right determination factor 1
Description of TargetLeft right determination factor 1
Uniprot IDD4A670
Protein Accession #NP_001102550
Nucleotide Accession #NM_001109080
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