LEFTY1 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03712)

Catalog No: OKCD03712

Size:96WELLS

Mouse LEFTY1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	78-96%	82-97%	91-101%
  EDTA Plasma (n=5)	93-101%	97-105%	94-102%	94-102%
  Heparin Plasma (n=5)	86-99%	80-104%	78-93%	78-94%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-91	87
  EDTA Plasma (n=5)	81-98	89
  Heparin Plasma (n=5)	80-95	79

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Lefty1 Microplate: 96 Wells (12 x 8 Well strips)
  • Lefty1 Lyophilized Standard: 2
  • 100X Lefty1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Lefty1
• Gene Full Name: left right determination factor 1
• Alias Symbols: AI450052;Eba;Ebaf;Left;Leftb;left-right determination factor 1;left-right determination, factor B;lefty;lefty-;lefty-1;protein lefty-1;St;stimulated by retinoic acid gene 3 protein;Stra3;Tgfb4;TGF-beta-4;transforming growth factor beta-4.
• NCBI Gene Id: 13590
• Protein Name: Left-right determination factor 1
• Description of Target: Required for left-right axis determination as a regulator of LEFTY2 and NODAL.
• Uniprot ID: Q64280
• Protein Accession #: NP_034224
• Nucleotide Accession #: NM_010094