LEF1 Antibody (OAEG00130)

Catalog No: OAEG00130

Size:100ug

Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF-1. Cells were stained with Aviva's LEF-1 antibody, clone REMB1 and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.08% sodium azide
• Clonality: Monoclonal
• Clone: REMB1
• Isotype: IgG1
• Host: Mouse
• Application: WB, IF
  Positive Controls: Jurkat cell lysate
• Reconstitution and Storage: Shipped at ambient temperature, freeze upon arrival. Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
• Immunogen: Full length recombinant LEF-1 protein.
• Purification: Protein A/G Chromatography
• Concentration: 1 mg/ml
• Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 – 1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Application Info: WB: 1-5 ug/mL
  IF: 1 ug/mL

Target Info

• Gene Symbol: LEF1
• Gene Full Name: Lymphoid enhancer-binding factor 1
• Alias Symbols: LEF-1, TCF10, TCF7L3, TCF1ALPHA
• NCBI Gene Id: 51176
• Description of Target: The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB1 recognizes the b-catenin binding domain of LEF-1.
• Uniprot ID: Q9UJU2
• Protein Accession #: NP_001124185.1; NP_001124186.1; NP_001159591.1; NP_057353.1
• Nucleotide Accession #: NM_001130713.2; NM_001130714.2; NM_001166119.1; NM_016269.4