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LEF1 Antibody (OAEB01737)

100 ug
$349.00
In Stock
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Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality:
Polyclonal
Host:
Goat
Application:
WB
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Gene Symbol:
LEF1
NCBI Gene Id:
51176
Protein Name:
Lymphoid enhancer-binding factor 1
Swissprot Id:
Q9UJU2
Protein Accession #:
NP_057353.1
Alias Symbols:
LEF1, lymphoid enhancer-binding factor 1, HGNC:6551, DKFZp586H0919, TCF1ALPHA, lymphoid enhancer binding factor-1
Replacement Item:
This antibody may replace item sc-28687 from Santa Cruz Biotechnology.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express LEF1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express LEF1.
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
Complete computational species homology data:
LEF1 Antibody (OAEB01737)
Peptide Sequence:
C-QHEQRKEQEPKRPH
Datasheets/Manuals:
Printable datasheet for anti-LEF1 antibody - OAEB01737
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 48kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al, EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). Recommended concentration: 1-3ug/ml.
Target Reference:
* Radtke F, Clevers H.; Self-renewal and cancer of the gut: two sides of a coin.; Science. 2005 Mar 25;307(5717):1904-9. Review.; Pubmed 15790842

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