Catalog No: OKCD01683 (Formerly GWB-KBBRD2)
Size:96WELLS
Price: $700.00
SKU
OKCD01683
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Datasheets/ManualsPrintable datasheet for Lect1 ELISA Kit (Mouse) (OKCD01683)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)81-99%81-96%86-98%96-104%
EDTA Plasma (n=5)85-99%81-91%97-104%82-94%
Heparin Plasma (n=5)97-105%93-102%84-103%79-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Cell Derived Chemotaxin 1 (LECT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukocyte Cell Derived Chemotaxin 1 (LECT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukocyte Cell Derived Chemotaxin 1 (LECT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukocyte Cell Derived Chemotaxin 1 (LECT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-10388
EDTA Plasma (n=5)87-9591
Heparin Plasma (n=5)90-10595
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukocyte Cell Derived Chemotaxin 1 (LECT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukocyte Cell Derived Chemotaxin 1 (LECT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Lect1 Microplate96 Wells (12 x 8 Well Strips)
Lect1 Lyophilized Standard2 x 2 ng
100X Biotinylated Lect1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 11.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Leukocyte Cell Derived Chemotaxin 1 (LECT1). No significant cross-reactivity or interference between Leukocyte Cell Derived Chemotaxin 1 (LECT1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCnmd
Gene Full Namechondromodulin
Alias SymbolsBric;Bricd3;ChM-;Chmd;ChM-I;Chondromodulin;Chondromodulin 1;chondromodulin-1;chondromodulin-I;Lect;Lect1;leukocyte cell derived chemotaxin 1;leukocyte cell-derived chemotaxin 1.
NCBI Gene Id16840
Protein NameLeukocyte cell-derived chemotaxin 1
Description of TargetBifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development (By similarity). Inhibits in vitro tube formation and mobilization of endothelial cells (By similarity). Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization.
Uniprot IDQ9Z1F6
Protein Accession #NP_001297584.1
Nucleotide Accession #NM_001310655.1
Protein Size (# AA)334
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