LECT1 Antibody - C-terminal region (OAAB01408)

Catalog No: OAAB01408

Size:400ul

Western blot analysis of anti-LECT1 Antibody (C-term) in NCI-H460 cell lysates (35ug/lane).LECT1(arrow) was detected using the purified Pab.

Western blot analysis of LECT1 (arrow) using rabbit polyclonal LECT1 Antibody (C-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the LECT1 gene.

LECT1 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with LECT1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse, Rat, Bovine, Rabbit
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Function: Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development. Inhibits in vitro tube formation and mobilization of endothelial cells. Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization.
  Cellular Location: Chondromodulin-1: Secreted, extracellular space, extracellular matrix. Note=Accumulated in the inter- territorial matrix of cartilage.
  Tissue Location: Detected in cartilage and cardiac valves (at protein level). Detected in the laminae fibrosa, spongiosa and ventricularis layers of normal cardiac valves (at protein level) Expression is decreased cardiac valves of patients with valvular heart disease (at protein level). Weakly expressed in chondrosarcoma.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This LECT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the C-terminal region of human LECT1.
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sample Type Confirmation:

  LECT1 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: IHC-P: 1:10~50
  WB: 1:1000
  Optimal dilutions should be determined by the end user.
• Reference: Aoyama,T., Biochem. Biophys. Res. Commun. 365 (1), 124-130 (2008)
  Yoshioka,M., Nat. Med. 12 (10), 1151-1159 (2006)
  Aoyama,T., J. Biol. Chem. 279 (27), 28789-28797 (2004)

Target Info

• Gene Symbol: LECT1
• Alias Symbols: CHM1, CHM-I, LECT1, BRICD3, MYETS1
• NCBI Gene Id: 11061
• Protein Name: Leukocyte cell-derived chemotaxin 1
• Description of Target: LECT1 a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This protein is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development.
• Uniprot ID: O75829
• Protein Accession #: NP_001011705.1
• Molecular Weight: 37 kDa