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Catalog No: OKEH03706
Size:96T
Price: $525.00
SKU
OKEH03706
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LDL ELISA Kit (Rat) (OKEH03706) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LDL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for LDL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LDL captured in well.
ELISA Kit Range6.25-400ng/mL
ELISA Kit Component
ComponentAmount
LDL Microplate96 Wells (12 x 8 Well strips)
LDL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated LDL Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity3.165 ng/mL
SpecificityNatural and recombinant Rat Low-density lipoprotein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLDL
Gene Full NameLow-density lipoprotein
Alias SymbolsLow-density lipoprotein
Protein NameLow-density lipoprotein
Description of TargetLow-density lipoprotein (LDL) is one of the five major groups of lipoproteins, which in order of size, largest to smallest, are chylomicrons, VLDL, IDL, LDL, and HDL, that enable transport of multiple different fat molecules, including cholesterol, within the water around cells and within the water-based bloodstream.
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