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Catalog No: OKEH03475
Size:96T
Price: $525.00
SKU
OKEH03475
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LDHB ELISA Kit (Rat) (OKEH03475) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ldhb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ldhb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ldhb captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Component
ComponentAmount
Anti-Ldhb Microplate96 Wells (12 x 8 Well strips)
Ldhb Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Ldhb Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.786 ng/mL
SpecificityNatural and recombinant Rat L-lactate dehydrogenase B chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLdhb
Gene Full Namelactate dehydrogenase B
Alias SymbolsLactate dehydrogenease B;LDH heart subunit;Ldh2;LDH-B;LDH-H;L-lactate dehydrogenase B chain.
NCBI Gene Id24534
Protein NameP42123
Description of TargetL-lactate dehydrogenase B chain
Uniprot IDhttps://www.uniprot.org/uniprot/P42123
Protein Accession #NP_001303262.1
Nucleotide Accession #NM_001316333.1
Protein Size (# AA)334
Molecular Weight37 kDa
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