LDHA ELISA Kit (Mouse) (OKCA02404)

Catalog No: OKCA02404

Size:96 Wells

Mouse LDHA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  1:100	100	95-106
  1:200	88	84-93
  1:400	107	103-110
  1:800	86	83-90

• ELISA Kit Principle: Aviva Systems Biology Ldha ELISA Kit (Mouse) (OKCA02404) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Ldha. Sample or standards are added to the wells along with a fixed quantity of HRP-Conjugated Ldha Detector Antibody and incubated. The Ldha found in the sample or standards competes with immobilized Ldha for binding with the HRP-Conjugated Ldha Detector Antibody. Wells are washed and an enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product. Addition of acidic stop solution changes the coloration to yellow. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ldhacaptured in the well and inversely proportional to the amount of Ldha which was contained in the sample or standard.
• ELISA Kit Range: 0.156 - 10 mU/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	101	96-108
  EDTA Plasma (n=4)	85	81-90

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Ldha Microplate: 96 Wells (12 x 8 Well strips)
  • Ldha Lyophilized Standard: 2
  • 100X HRP Conjugated Ldha Detector Anitbody: 1 x 60 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Sample Diluent: 2 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.039 mU/mL
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition Immunoassay

Target Info

• Gene Symbol: LDHA
• Alias Symbols: l7, LDH, Ldh-, Ldh1, Ldhm, l7R2
• NCBI Gene Id: 16828
• Protein Name: L-lactate dehydrogenase A chain
• Uniprot ID: P06151
• Protein Accession #: NP_034829
• Nucleotide Accession #: NM_010699