Catalog No: OKCA02404
Size:96 Wells
Price: $857.00
SKU
OKCA02404
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse | ||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Ldha ELISA Kit (Mouse) (OKCA02404) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Ldha. Sample or standards are added to the wells along with a fixed quantity of HRP-Conjugated Ldha Detector Antibody and incubated. The Ldha found in the sample or standards competes with immobilized Ldha for binding with the HRP-Conjugated Ldha Detector Antibody. Wells are washed and an enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product. Addition of acidic stop solution changes the coloration to yellow. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ldhacaptured in the well and inversely proportional to the amount of Ldha which was contained in the sample or standard. | ||||||||||||||||||
ELISA Kit Range | 0.156 - 10 mU/mL | ||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay: CV <12% (n=20) Mean Inter-Assay: CV <15% (n=24) | ||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||
Sensitivity | 0.039 mU/mL | ||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive Inhibition Immunoassay |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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