LDCA Recombinant Protein (Escherichia coli) (OPCA03989)

Catalog No: OPCA03989

Product Info

• Predicted Species Reactivity: Escherichia coli
• Product Format: Liquid
• Additional Information: Species Specificity Detail: Escherichia coli (strain K12)
• Reconstitution and Storage: Briefly centrifuge lyophilized product prior to opening to bring the contents to the bottom. Please reconstitute protein to 0.1-1.0 mg/mL by adding deionized sterile water first, followed by addition of glycerol to a final concentration of 5-50%. Reconstituted product should be aliquoted for long-term storage at -20C/-80C. Our in house default final concentration of glycerol is 50% for reference. The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
• Formulation: Tris-base, 50% glycerol
• Purity: Greater than 90% as determined by SDS-PAGE.
• Protein Sequence: Full Length: MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQGEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILNNTREGTQLTISGHPVLKM
• Storage Buffer: Tris-base, 50% glycerol
• Source: E.coli
• Tag: N-terminal 6xHis-tagged
• Reference: A defect in cell wall recycling triggers autolysis during the stationary growth phase of Escherichia coli.Templin M.F., Ursinus A., Hoeltje J.-V.EMBO J. 18:4108-4117(1999)

Target Info

• Gene Symbol: LDCA
• Alias Symbols: ECK1180, ycgQ
• NCBI Gene Id: 945756
• Protein Name: Murein tetrapeptide carboxypeptidase
• Description of Target: Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso-Dap-D-Ala. To a lesser extent, can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc-tetrapeptide, GlcNAc-MurNAc-tetrapeptide, and GlcNAc-anhMurNAc-tetrapeptide. Does not act on murein sacculi or cross-linked muropeptides. The tripeptides produced by the LcdA reaction can then be reused as peptidoglycan building blocks; LcdA is thereby involved in murein recycling. Is also essential for viability during stationary phase.
• Uniprot ID: P76008
• Protein Accession #: NP_415710
• Nucleotide Accession #: NC_000913
• Molecular Weight: 38 kDa