LDB1 Antibody (OAGA00317)

Catalog No: OAGA00317

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
OAGA00317 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
OAGA00317 diluted at 1:1000

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
LDB1 antibody [N2C3] (OAGA00317) dilution: 1:500.

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
LDB1 antibody [N2C3] (OAGA00317) dilution: 1:500.

Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(OAGA00317) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using LDB1(OAGA00317) antibody at 1:200 dilution.

LDB1antibody immunoprecipitates LDB1 protein in IP experiments.
IP Sample: 1000 ug 293T whole cell lysate/extract
A. 20 ?g 293T whole cell lysate/extract
B. Control with 2.5 ?g of preimmune rabbit IgG
C. Immunoprecipitation of LDB1 protein by 2.5 ?g of LDB1 antibody (OAGA00317)
12% SDS-PAGE
The immunoprecipitated LDB1 protein was detected by LDB1antibody (OAGA00317) diluted at 1:1000.
EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, IP, WB
• Additional Information: Background: Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
  Positive Controls: 293T, A431, HeLa, HepG2, mouse brain
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 86 and 411 of LDB1 (Uniprot ID#Q86U70)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.93 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: LDB1
• Gene Full Name: LIM domain-binding protein 1
• Alias Symbols: NLI, CLIM2, LDB-1, CLIM-2
• NCBI Gene Id: 8861
• Protein Name: LIM domain-binding protein 1
• Uniprot ID: Q86U70
• Protein Accession #: NP_001106878.1
• Molecular Weight: 43 kDa