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Catalog No: OKDD00372 (Formerly GWB-KBBYY1)
Size:96 Wells
Price: $700.00
SKU
OKDD00372
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Datasheets/ManualsPrintable datasheet for LCP1 ELISA Kit (Human) (OKDD00372)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology LCP1 ELISA Kit (Human) (OKDD00372) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for LCP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCP1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LCP1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LCP1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LCP1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-LCP1 Microplate96 Wells (12 x 8 Well Strips)
LCP1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X LCP1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of LCP1. No significant cross-reactivity or interference between LCP1 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolLCP1
Gene Full Namelymphocyte cytosolic protein 1
Alias SymbolsbA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin));CP64;epididymis secretory protein Li 37;HEL-S-37;LC64P;LCP-1;LPL;L-PLASTIN;L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P);Lymphocyte cytosolic protein 1;Lymphocyte cytosolic protein-1 (plasmin);plastin-2;PLS2.
NCBI Gene Id3936
Protein NamePlastin-2
Description of TargetActin-binding protein (PubMed:16636079, PubMed:17294403, PubMed:28493397). Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Modulates the cell surface expression of IL2RA/CD25 and CD69 (PubMed:17294403).
Uniprot IDP13796
Protein Accession #NP_002289.2
Nucleotide Accession #NM_002298.4
Protein Size (# AA)627
Molecular Weight70 kDa
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