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Catalog No: OKEH04085
Size:96T
Price: $425.00
SKU
OKEH04085
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Lcn2 ELISA Kit (Mouse) (OKEH04085) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lcn2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lcn2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lcn2 captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.5% (n = 20)
Mean Inter-assay CV%: <= 9.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Lcn2 Microplate96 Wells (12 x 8 Well Strips)
Lcn2 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Lcn2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.9 pg/mL
SpecificityNatural and recombinant Mouse Neutrophil gelatinase-associated lipocalin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLcn2
Gene Full Namelipocalin 2
Alias Symbols24p;24p3;AW212229;Lipocalin-2;N;neu-related lipocalin;neutrophil gelatinase-associated lipocalin;NRL;oncogene 24p3;p25;secreted inducible protein 24;siderocalin LCN2;Sip24;SV-40-induced 24P3 protein.
NCBI Gene Id16819
Protein NameP11672
Description of TargetNeutrophil gelatinase-associated lipocalin
Uniprot IDhttps://www.uniprot.org/uniprot/P11672
Protein Accession #NP_032517.1
Nucleotide Accession #NM_008491.1
Protein Size (# AA)200
Molecular Weight23 kDa
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