LCN15 ELISA Kit (Human) (OKCA01335)

Catalog No: OKCA01335

Size:96 Wells

Human LCN15 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	92	88-96
  1:2	90	86-97
  1:4	96	90-102
  1:8	94	91-98

• ELISA Kit Principle: Aviva Systems Biology LCN15 ELISA Kit (Human) (OKCA01335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LCN15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCN15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LCN15 captured in the well.
• ELISA Kit Range: 18.75 - 1,200 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	95	89-99
  EDTA Plasma (n=4)	87	85-96

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01335 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01335 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • LCN15 Microplate: 96 Wells (12 x 8 Well strips)
  • LCN15 Lyophilized Standard: 2 x 1.2 ng
  • 100X Biotinylated LCN15 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 4.68 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: LCN15
• Gene Full Name: lipocalin 15
• Alias Symbols: PRO6093, UNQ2541
• NCBI Gene Id: 389812
• Protein Name: Lipocalin-15
• Uniprot ID: Q6UWW0
• Protein Accession #: NP_976222.1
• Nucleotide Accession #: NM_203347.1