LCN1 ELISA Kit (Human) (OKCD07614)

Catalog No: OKCD07614

Size:96WELLS

LCN1 ELISA Kit (Human) (OKCD07614) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipocalin 1 (LCN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipocalin 1 (LCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipocalin 1 (LCN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipocalin 1 (LCN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipocalin 1 (LCN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipocalin 1 (LCN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LCN1 Microplate: 96 Wells (12 x 8 Well strips)
  • LCN1 Lyophilized Standard: 2
  • 100X LCN1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, saliva, tears and other biological fluids and other biological fluids
• Sensitivity: < 0.62ng/mL

Target Info

• Gene Symbol: LCN1
• Gene Full Name: lipocalin 1
• Alias Symbols: lipocalin-1;PMFA;protein migrating faster than albumin;Tear lipocalin;tear prealbumin;TLC;TP;VEG protein;VEGP;Von Ebner gland protein.
• NCBI Gene Id: 3933
• Protein Name: Lipocalin-1
• Description of Target: Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket.
• Uniprot ID: P31025
• Protein Accession #: NP_001239546.1
• Nucleotide Accession #: NM_001252617.1