LCK Polyclonal Antibody (Human) (ATP00010_T100)

Catalog No: ATP00010_T100

Size:100uL

20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent (OKCF00002).

Immunogen: Recombinant protein representing aa 2-240 of human Lck (green) depicted within in the full length structure prediction (blue)  via AlphaFold. (https://alphafold.ebi.ac.uk/).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: Recombinant Human LCK aa 2-240
• Purification: Protein A Purified
• Concentration: 1mg/mL

Target Info

• Alias Symbols: LSK;YT16;IMD22;p56lck;pp58lck
• NCBI Gene Id: 3932
• Protein Name: Tyrosine-protein kinase Lck
• Description of Target: This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]
• Uniprot ID: P06239-1
• Protein Accession #: P06239-1
• Molecular Weight: 58 kDa