LCAT ELISA Kit (Pig) (OKEH08482)

Catalog No: OKEH08482

Size:96T

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	97-106% (n = 20)	96-106% (n = 20)	91-101% (n = 20)	100-112% (n = 20)
  EDTA plasma(n=5)	103-113% (n = 20)	91-99% (n = 20)	99-108% (n = 20)	94-104% (n = 20)
  heparin plasma(n=5)	97-107% (n = 20)	92-101% (n = 20)	102-112% (n = 20)	113-123% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology LCAT ELISA Kit (Pig) (OKEH08482) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LCAT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LCAT captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.3% (n = 20)
  Mean Inter-assay CV%: <=4.8% (n = 20)
• ELISA Kit Component:
  • LCAT Microplate: 96 Wells (12 x 8 Well strips)
  • LCAT Lyophilized Standard: 2
  • 100X Biotinylated LCAT Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (By similarity). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107).
  Subcellular Location: Secreted Secreted into blood plasma (PubMed:8820107). Produced in astrocytes and secreted into cerebral spinal fluid (CSF) (By similarity).
  Unigene: Ssc.2393
  Pfam: PF02450
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Pig Phosphatidylcholine-sterol acyltransferase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LCAT
• Alias Symbols: Lecithin-cholesterol acyltransferase;Phospholipid-cholesterol acyltransferase.
• Protein Name: P30930
• Description of Target: Phosphatidylcholine-sterol acyltransferase
• Uniprot ID: https://www.uniprot.org/uniprot/P30930