LCAT ELISA Kit (Mouse) (OKEH07035)

Catalog No: OKEH07035

Size:96T

Mouse LCAT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Lcat ELISA Kit (Mouse) (OKEH07035) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lcat has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lcat is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lcat captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 89.4%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <7.6%
  Inter-Assay CV <9.5%
• ELISA Kit Component:
  • Lcat Microplate: 96 Wells (12 x 8 Well strips)
  • Lcat Lyophilized Standard: 2 x 10 ng
  • 100X Lcat HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Mouse Phosphatidylcholine-sterol acyltransferase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Lcat
• Gene Full Name: lecithin cholesterol acyltransferase
• Alias Symbols: 1-alkyl-2-acetylglycerophosphocholine esterase;AI046659;D8Wsu61;D8Wsu61e;Lecithin-cholesterol acyltransferase;lecithin-cholesterol acyltransferase Lcat;PAF acetylhydrolase;phosphatidylcholine-sterol acyltransferase;phospholipid-cholesterol acyltransferase;platelet-activating factor acetylhydrolase.
• NCBI Gene Id: 16816
• Protein Name: P16301
• Description of Target: Phosphatidylcholine-sterol acyltransferase
• Uniprot ID: https://www.uniprot.org/uniprot/P16301
• Protein Accession #: NP_032516
• Nucleotide Accession #: NM_008490