Catalog No: OKCD03708
Size:96WELLS
Price: $800.00
SKU
OKCD03708
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LCAT Chemi-Luminescent ELISA Kit (Human) (OKCD03708)

Datasheets/ManualsPrintable datasheet for LCAT Chemi-Luminescent ELISA Kit (Human) (OKCD03708)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)81-96%91-104%80-90%81-105%
EDTA Plasma (n=5)78-103%96-105%94-105%93-101%
Heparin Plasma (n=5)81-90%90-104%90-97%98-105%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lecithin Cholesterol Acyltransferase (LCAT) level in the sample or standard.
ELISA Kit Range0.21-150ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-105102
EDTA Plasma (n=5)78-10194
Heparin Plasma (n=5)80-8883
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
LCAT Microplate96 Wells (12 x 8 Well strips)
LCAT Lyophilized Standard2
100X LCAT Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity2.35 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLCAT
Gene Full Namelecithin-cholesterol acyltransferase
Alias Symbols1-alkyl-2-acetylglycerophosphocholine esterase;Lecithin-cholesterol acyltransferase;PAF acetylhydrolase;phosphatidylcholine-sterol acyltransferase;phosphatidylcholine--sterol O-acyltransferase;phospholipid-cholesterol acyltransferase;platelet-activating factor acetylhydrolase;testicular secretory protein Li 24.
NCBI Gene Id3931
Protein NamePhosphatidylcholine-sterol acyltransferase
Description of TargetCentral enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:10329423, PubMed:19065001, PubMed:26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:10722751).
Uniprot IDP04180
Protein Accession #NP_000220
Nucleotide Accession #NM_000229
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