MAP1LC3B Antibody - N-terminal region (OAAB13469)

Catalog No: OAAB13469

Size:200UL

Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #OAAB13469) in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 (APG8b) were detected in soluble fraction (S).

Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #OAAB13469) in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified Pab.

Fluorescent image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine (50 ?M, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Product Info

• Predicted Species Reactivity: Bovine, Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: Immunofluorescence, Immunohistochemistry-Paraffin, Western blot
• Additional Information: Precautions: LC3 Antibody (APG8B) (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Involved in formation of autophagosomal vacuoles (autophagosomes)
  Cellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes
  Tissue Location: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IF: 1:100
  IHC-P: 1:50~100

Target Info

• Gene Symbol: MAP1LC3B
• Gene Full Name: microtubule associated protein 1 light chain 3 beta
• Alias Symbols: ATG8F;Autophagy-related protein LC3 B;autophagy-related ubiquitin-like modifier LC3 B;LC3B;MAP1 light chain 3-like protein 2;MAP1A/1BLC3;MAP1A/MAP1B LC3 B;MAP1A/MAP1B light chain 3 B;MAP1LC3B-a;Microtubule-associated protein 1 light chain 3 beta;microtubule-associated proteins 1A/1B light chain 3B;Microtubule-associated proteins 1A/1B light chain 3B-like.
• NCBI Gene Id: 81631
• Protein Name: A6NCE7, NP_073729.1, O41515, Q9CQV6, Q9GZQ8
• Description of Target: Microtubule-associated proteins 1A/1B light chain 3 beta 2, Microtubule-associated proteins 1A/1B light chain 3B, microtubule-associated proteins 1A/1B light chain 3B [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/?query=A6NCE7+OR+O41515+OR+Q9CQV6+OR+Q9GZQ8
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_073729.1
• Molecular Weight: 14688