Catalog No: OPPA01425 (Formerly GWB-847651)
Size:2UG
Price: $233.00
SKU
OPPA01425
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPPA01425 |
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Predicted Species Reactivity | Mouse |
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Product Format | Recombinant Mouse LBP was lyophilized from a protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
Host | Chinese Hamster Ovarian Cells (CHO) |
Application | ELISA, WB |
Additional Information | Solubility: It is recommended to reconstitute the lyophilized LBP in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Product Introduction: Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein (BPI), LBP binds LPS and interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, and to a binding site on soluble CD14 (sCD14). sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, and then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid. Product Description: LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page. Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off. up to 2 ug/ml LBP mediates binding of FITC-LPS (0.5 ug/ml) to CD14+CHO transfectants (2 x 1,000,000/ml). |
Reconstitution and Storage | Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution LBP should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles. |
Application Info | Characterization: On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa. |
Gene Symbol | mLBP |
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Alias Symbols | Bpif, Ly88, Bpifd2 |
NCBI Gene Id | 16803 |
Protein Name | Lipopolysaccharide-binding protein |
Description of Target | Recombinant Mouse Lipopolysaccarid Binding Protein |
Uniprot ID | Q61805 |
Protein Accession # | NP_032515.2 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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