LBP ELISA Kit (Rat) (OKCA00979)

Catalog No: OKCA00979

Size:96 Wells

Rat LBP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  1:5	85	81-89
  1:10	94	91-98
  1:20	91	85-97
  1:40	86	82-89

• ELISA Kit Principle: Aviva Systems Biology LBP ELISA Kit (Rat) (OKCA00979) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for LBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LBP captured in well.
• ELISA Kit Range: 62.5 pg/mL-4,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	100	97-103
  EDTA Plasma (n=4)	94	90-98

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level LBP were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level LBP were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • LBP Microplate: 96 Wells (12 x 8 Well strips)
  • LBP Lyophilized Standard: 2
  • 100X Biotinylated LBP Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LBP
• Gene Full Name: Lipopolysaccharide-binding protein
• Protein Name: Lipopolysaccharide-binding protein
• Description of Target: Plays a role in the innate immune response . Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria . Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide .
• Uniprot ID: P17454
• Protein Accession #: NP_001182648.1
• Nucleotide Accession #: NM_001195719.1