LBP ELISA Kit (Human) (OKBB00931)

Catalog No: OKBB00931

Size:96 wells

LBP ELISA Kit (Human) (OKBB00931) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for LBP has been precoated onto 96-well plates. Standards(NS0, A26-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human LBP amount of sample captured in plate.
• ELISA Kit Range: 0.78 ng/ml - 50 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	1485	4533	23562	1518	4575	21374
  Standard deviation	66.82	271.98	1507.96	85	297.37	1432.05
  CV(%)	4.5	6	6.4	5.6	6.5	6.7

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human LBP Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human LBP Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human LBP Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	1485	1561	1630	1640	1579	62.21	3.9%
  Sample 2	4533	5189	4438	4703	4715	289.25	6.1%
  Sample 3	23562	22985	21907	22035	22622	683.95	3%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
  O.D.	0.096	0.147	0.218	0.358	0.591	0.913	1.407	2.090

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <50 pg/ml
• Specificity: Natural and recombinant human LBP
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A26-V481
• Description: For quantitative detection of human LBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: LBP
• Gene Full Name: lipopolysaccharide binding protein
• Alias Symbols: BPI fold containing family D, member 2, Bpifd2, LBP, LBP_HUMAN, LBP1, Lipopolysaccharide binding protein, Lipopolysaccharide-binding protein, LPS binding protein, Ly88, MGC22233, OTTHUMP00000030965, RP23-407H16.4
• NCBI Gene Id: 3929
• Protein Name: Lipopolysaccharide-binding protein
• Description of Target: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
• Uniprot ID: P18428
• Protein Accession #: NP_004130.2
• Nucleotide Accession #: NM_004139.4