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LBP ELISA Kit (Bovine) (OKCD01154)

Catalog#: OKCD01154
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
This product is Genway GWB-KBBCB8
More Information
Predicted Species Reactivity Bovine
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Duration 2.5 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
EDTA plasma(n=5)78-99%80-93%95-102%79-93%
heparin plasma(n=5)81-95%92-101%83-97%83-105%
Kit Principle Aviva Systems Biology LBP ELISA Kit (Bovine) (OKCD01154) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LBPcaptured in well.
Kit Range 0.625 - 40 ng/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
EDTA plasma(n=5)88-10393
heparin plasma(n=5)85-9489
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level LBP were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level LBP were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Component
Anti-LBP Microplate96 Wells (12 x 8 Well Strips)
LBP Lyophilized Standard2 x 40 ng
100X Biotinylated LBP Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma and other biological fluids
Sensitivity < 0.291 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of Lipopolysaccharide Binding Protein (LBP). No significant cross-reactivity or interference between Lipopolysaccharide Binding Protein (LBP) and analogues was observed.
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Gene Symbol LBP
Official Gene Full Name lipopolysaccharide binding protein
Alias Symbols Lipopolysaccharide-binding protein; LBP
NCBI Gene Id 512242
Protein Name lipopolysaccharide-binding protein
Description of Target Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Swissprot Id Q2TBI0
Protein Accession # NP_001121907
Nucleotide Accession # NM_001128435.1
Protein Size (# AA) 481
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express LBP.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express LBP.
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