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Catalog No: OKCA02041
Size:96 Wells
Price: $857.00
SKU
OKCA02041
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBovine
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
Sample TypeDilutionMean (%)Range (%)
Serum (n=4)Neat9284-102
Serum (n=4)1:28682-95
Serum (n=4)1:49890-107
Serum (n=4)1:89590-100
ELISA Kit PrincipleAviva Systems Biology LBP ELISA Kit (Bovine) (OKCA02041) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LBP captured in well.
ELISA Kit Range15.6 ng/mL-1,000 ng/mL
ELISA Kit Recovery
Sample TypeMean (%)Range (%)
Serum (n=5)9288-96
EDTA plasma (n=4)9590-101
ELISA Kit ReproducibilityMean Intra-Assay Variation <10% CV (n=20)
Mean Inter-Assay Variation <12% CV (n=8)
ELISA Kit Component
ComponentAmount
Anti-LBP Microplate96 Wells (12 x 8 Well strips)
LBP Lyophilized Standard 2 x 1 ug
100X Biotinylated LBP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity3.9 ng/mL
Publications

Seasonal heat load is more potent than the degree of body weight loss in dysregulating immune function by reducing white blood cell populations and increasing inflammation in Holstein dairy cows. J Dairy Sci. 103, 10809-10822 (2020). 32896401

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolLBP
Alias SymbolsLipopolysaccharide-binding protein
NCBI Gene Id512242
Protein NameLipopolysaccharide-binding protein
Description of TargetPlays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Uniprot IDQ2TBI0
Protein Accession #NP_001033763
Nucleotide Accession #NM_001038674
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