LATS1 Antibody - N-terminal region (OAAB16771)

Catalog No: OAAB16771

Size:400ul

Western blot analysis of anti-LATS1 Antibody (N-term) (Cat.# OAAB16771) in K562 cell line lysates (35ug/lane). LATS1 (arrow) was detected using the purified Pab.

LATS1 is strongly supported by BioGPS gene expression data to be expressed in K562

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC-P, WB
• Additional Information: Precautions: LATS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function
  Cellular Location: Cytoplasm, cytoskeleton, centrosome. Note=Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis
  Tissue Location: Expressed in all adult tissues examined except for lung and kidney
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LATS1.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  LATS1 is strongly supported by BioGPS gene expression data to be expressed in K562

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: LATS1
• Alias Symbols: wts, WARTS
• NCBI Gene Id: 9113
• Protein Name: Serine/threonine-protein kinase LATS1
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O95835
• Protein Accession #: NP_001257448.1
• Molecular Weight: 126870 Da