LATS1/2 Antibody (OAAF01525)

Catalog No: OAAF01525 (Formerly GWB-ASC895)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using LATS1/2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human LATS1/2.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: LATS1/2 antibody detects endogenous levels of total LATS1/2 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Tao W., Nat. Genet. 21:177-181(1999).
  Nishiyama Y., FEBS Lett. 459:159-165(1999).
  The MGC Project Team, Genome Res. 14:2121-2127(2004).

Target Info

• Gene Symbol: LATS1|LATS2
• Gene Full Name: large tumor suppressor kinase 1|large tumor suppressor kinase 2
• Alias Symbols: h-warts;kinase phosphorylated during mitosis protein;KPM;large tumor suppressor homolog 1;large tumor suppressor homolog 2;LATS (large tumor suppressor, Drosophila) homolog 1;LATS (large tumor suppressor, Drosophila) homolog 2;LATS, large tumor suppressor, homolog 1;LATS, large tumor suppressor, homolog 2;serine/threonine kinase KPM;serine/threonine-protein kinase kpm;serine/threonine-protein kinase LATS1;serine/threonine-protein kinase LATS2;WARTS;WARTS protein kinase;warts-like kinase;wts.
• NCBI Gene Id: 26524|9113
• Protein Name: Serine/threonine-protein kinase LATS1|Serine/threonine-protein kinase LATS2
• Description of Target: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed:28068668).|Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.
• Uniprot ID: O95835|Q9NRM7
• Protein Accession #: NP_055387.2
• Molecular Weight: 126 kDa