LASS1 Antibody (OAEB02140)

Catalog No: OAEB02140 (Formerly GWB-EV0349)

Size:100UG

OAEB02140 (1ug/ml) staining of Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Porcine
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CERS1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat
• Peptide Sequence: C-KDLREYDTAEAQ
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Approx. 37 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 38.0 kDa according to NP_937850.1). Recommended concentration: 1-3ug/ml.
  An additional band of unknown identity was also consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide.
• Reference: * Venkataraman K, Riebeling C, Bodennec J, Riezman H, Allegood JC, Sullards MC, Merrill AH Jr, Futerman AH.; Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells.; J Biol Chem. 2002 Sep 20;277(38):35642-9. Epub 2002 Jun 24.; Pubmed 12105227

Target Info

• Gene Symbol: Cers1|CERS1|Gdf1
• Gene Full Name: ceramide synthase 1|growth differentiation factor 1
• Alias Symbols: Cer;ceramide synthase 1;Embryonic growth/differentiation factor 1;EPM8;GDF1;GDF-1;L;LAG1;LAG1 homolog, ceramide synthase 1;LAG1 longevity assurance homolog 1;LASS1;longevity assurance (LAG1, S. cerevisiae) homolog 1;longevity assurance gene 1 protein homolog 1;longevity assurance homolog 1;protein UOG-1;t;to;Uog-;Uog-1;UOG1;upstream of GDF1.
• NCBI Gene Id: 10715|306351|93898
• Protein Name: Ceramide synthase 1
• Uniprot ID: P27544
• Protein Accession #: NP_067090.1
• Molecular Weight: 39.5; 38.0