LARGE1 Antibody (OAGA05612)

Catalog No: OAGA05612

Size:100 ul

LARGE antibody detects LARGE protein by western blot analysis. Mouse tissue extracts (50 ug) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with LARGE antibody at a dilution of 1:1000.

LARGE antibody detects LARGE protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
LARGE antibody dilution: 1:500.

LARGE antibody detects LARGE protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
LARGE antibody dilution: 1:500.

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human LARGE. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: LARGE1
• Gene Full Name: LARGE xylosyl- and glucuronyltransferase 1
• Alias Symbols: LARGE, MDC1D, MDDGA6, MDDGB6
• NCBI Gene Id: 9215
• Protein Name: LARGE xylosyl- and glucuronyltransferase 1; glycosyltransferase-like protein LARGE1
• Description of Target: This gene, which is one of the largest in the human genome, encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. Mutations in this gene cause MDC1D, a novel form of congenital muscular dystrophy with severe cognitive disability and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in two transcript variants that encode the same protein.
• Uniprot ID: O95461
• Protein Accession #: NP_004728.1
• Nucleotide Accession #: NM_004737.5