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| Datasheets/Manuals | Printable datasheet for LARGE Antibody (OAEE00484) (OAEE00484) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Clonality | Monoclonal |
| Clone | LARGE-02 |
| Isotype | IgG2b |
| Host | Mouse |
| Application | Flow cytometry|Western blot |
| Reconstitution and Storage | 2°C to 8°C |
| Immunogen | Recombinant fragment of human LARGE1 (amino acids 35-142) |
| Purification | Purified |
| Concentration | 1 mg/ml |
| Specificity | The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase localizing mainly to the Golgi apparatus. Crossreactivity with LARGE2 was not determined. |
| Formulation | Purified |
| Purity | > 95% (by SDS-PAGE) |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Reference | Grewal PK, McLaughlan JM, Moore CJ, Browning CA, Hewitt JE: Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies. Glycobiology. 2005 Oct;15(10):912-23. |
| Gene Symbol | LARGE1 |
|---|---|
| Gene Full Name | LARGE xylosyl- and glucuronyltransferase 1 |
| Alias Symbols | acetylglucosaminyltransferase-like 1A;acetylglucosaminyltransferase-like protein;Glycosyltransferase-like protein;glycosyltransferase-like protein LARGE1;LARGE;LARGE xylosyl- and glucuronyltransferase 1;MDC1D;MDDGA6;MDDGB6. |
| NCBI Gene Id | 9215 |
| Protein Name | LARGE xylosyl- and glucuronyltransferase 1 |
| Description of Target | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699). |
| Uniprot ID | O95461 |
| Protein Accession # | NP_004728.1; NP_598397.1; XP_005261888.1; XP_005261889.1 |
| Nucleotide Accession # | NM_004737.4; NM_133642.3; XM_005261831.2; XM_005261832.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "LARGE Antibody (OAEE00484)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "LARGE Antibody (OAEE00484)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "LARGE Antibody (OAEE00484)" provided in?
This item is provided in "Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "LARGE Antibody (OAEE00484)"?
This target may also be called "acetylglucosaminyltransferase-like 1A;acetylglucosaminyltransferase-like protein;Glycosyltransferase-like protein;glycosyltransferase-like protein LARGE1;LARGE;LARGE xylosyl- and glucuronyltransferase 1;MDC1D;MDDGA6;MDDGB6." in publications.
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What is the shipping cost for "LARGE Antibody (OAEE00484)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "LARGE Antibody (OAEE00484)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "LARGE Antibody (OAEE00484)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "LARGE Antibody (OAEE00484)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "LARGE1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "LARGE1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "LARGE1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "LARGE1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "LARGE1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "LARGE1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
