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LARGE Antibody - N-terminal region (OAEB00400)

100 ug
$229.00
In Stock
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Gene Symbol:
LARGE
NCBI Gene Id:
9215
Protein Name:
Glycosyltransferase-like protein LARGE1
Swissprot Id:
O95461
Protein Accession #:
NP_004728.1
Alias Symbols:
LARGE, like-glycosyltransferase, KIAA0609, MDC1D, acetylglucosaminyltransferase-like 1A, acetylglucosaminyltransferase-like protein, like-acetylglucosaminyltransferase
Replacement Item:
This antibody may replace item sc-33435 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express LARGE.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express LARGE.
Predicted Species Reactivity:
Horse, Human, Mouse, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100%
Complete computational species homology data:
LARGE antibody - N-terminal region (OAEB00400)
Peptide Sequence:
RYTASSQRERESLE-C
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-LARGE antibody - OAEB00400
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Preliminary experiments gave an approx 125kDa band in Human Kidney, Pancreas and Placenta lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 88.1kDa according to NP_004728.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide.
Target Reference:
* de Bernabe DB, Inamori K, Yoshida-Moriguchi T, Weydert CJ, Harper HA, Willer T, Henry MD, Campbell KP,; Loss of alpha-dystroglycan laminin binding in epithelium-derived cancers is caused by silencing of LARGE.; The Journal of biological chemistry 2009 Apr 284 (17): 11279-84.; Pubmed 19244252

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