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Datasheets/Manuals | Printable datasheet for OASE00291-HRP |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Monoclonal |
Clone | S165-38 |
Isotype | IgG2a |
Host | Mouse |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB, ICC |
Additional Information | Background Info: Detects a 85 kDa protein (full length protein is 210 kDa – smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains. |
:: | Scientific Background: PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from beta-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function (1,2,3,4). Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide. |
:: | Certificate of Analysis: 1ug/mL was sufficient for detection of LAR/PTPRF in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR (accession # P10586). 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK. |
Purification | Protein G Purified |
Concentration | 0.6-0.7 mg/ml |
Dilution | 1:1000 WB |
Storage Buffer | PBS pH 7.4, 50% glycerol, 0.1% sodium azide |
Reference | 1. Streuli M. M., et al. (1992) EMBO Journal, 11(3), 897-907. 2. Mooney R.A. and C.M LeVea. (2003) Current Topics in Medicinal Chemistry, 3(7), 809-819. 3. Chagnon M.J., et al. (2004) Biochemistry and Cell Biology, 82(6), 664-675. 4. Ahmad F.F., and B.J. Goldstein (1997) Journal of Biological Chemistry, 272(1), 448-457. |
Gene Symbol | PTPRF |
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Gene Full Name | protein tyrosine phosphatase, receptor type, F |
Alias Symbols | LAR, BNAH2 |
NCBI Gene Id | 5792 |
Uniprot ID | P10586 |
Protein Accession # | NP_002831.2. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "LAR/PTPRF Antibody : HRP (OASE00291-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "LAR/PTPRF Antibody : HRP (OASE00291-HRP)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "LAR/PTPRF Antibody : HRP (OASE00291-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "LAR/PTPRF Antibody : HRP (OASE00291-HRP)"?
This target may also be called "LAR, BNAH2" in publications.
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What is the shipping cost for "LAR/PTPRF Antibody : HRP (OASE00291-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "LAR/PTPRF Antibody : HRP (OASE00291-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "LAR/PTPRF Antibody : HRP (OASE00291-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "LAR/PTPRF Antibody : HRP (OASE00291-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PTPRF"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PTPRF"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PTPRF"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PTPRF"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PTPRF"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PTPRF"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.