LAR/PTPRF Antibody : Biotin (OASE00291-Biotin)

Catalog No: OASE00291-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S165-38
• Isotype: IgG2a
• Host: Mouse
• Conjugation: Biotin
• Application: WB, ICC
• Additional Information: Background Info: Detects a 85 kDa protein (full length protein is 210 kDa – smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.
  Scientific Background: PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from beta-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function (1,2,3,4). Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.
  Certificate of Analysis: 1ug/mL was sufficient for detection of LAR/PTPRF in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR (accession # P10586). 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 WB
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Reference: 1. Streuli M. M., et al. (1992) EMBO Journal, 11(3), 897-907.
  2. Mooney R.A. and C.M LeVea. (2003) Current Topics in Medicinal Chemistry, 3(7), 809-819.
  3. Chagnon M.J., et al. (2004) Biochemistry and Cell Biology, 82(6), 664-675.
  4. Ahmad F.F., and B.J. Goldstein (1997) Journal of Biological Chemistry, 272(1), 448-457.

Target Info

• Gene Symbol: PTPRF
• Gene Full Name: protein tyrosine phosphatase, receptor type, F
• Alias Symbols: LAR, BNAH2
• NCBI Gene Id: 5792
• Uniprot ID: P10586
• Protein Accession #: NP_002831.2.