LAPTM4B ELISA Kit (Bovine) (OKEH07299)

Catalog No: OKEH07299

Size:96T

Bovine LAPTM4B ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology LAPTM4B ELISA Kit (Bovine) (OKEH07299) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAPTM4B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAPTM4B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAPTM4B captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.1% (n = 20)
  Mean Inter-assay CV%: <=10.0% (n = 20)
• ELISA Kit Component:
  • LAPTM4B Microplate: 96 Wells (12 x 8 Well strips)
  • LAPTM4B Lyophilized Standard: 2
  • 100X Biotinylated LAPTM4B Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
  
  Subcellular Location: Endomembrane system Multi-pass membrane protein
  Unigene: Bt.1143
  String: 9913.ENSBTAP00000022559
  KEGG: bta:404155
  Pfam: PF03821
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.078ng/mL
• Specificity: Natural and recombinant Bovine Lysosomal-associated transmembrane protein 4B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LAPTM4B
• Gene Full Name: lysosomal protein transmembrane 4 beta
• Alias Symbols: lysosomal associated protein transmembrane 4 beta;lysosomal-associated transmembrane protein 4B.
• NCBI Gene Id: 404155
• Protein Name: Q71SV0
• Description of Target: Lysosomal-associated transmembrane protein 4B
• Uniprot ID: https://www.uniprot.org/uniprot/Q71SV0
• Protein Accession #: NP_991371.1
• Nucleotide Accession #: NM_205802.1